Biolegend, 846202, Purified anti-PINK1 Antibody, 100μg

PINK1 (PTEN-induced putative kinase 1) is a 63 kD mitochondrial transmembrane protein that is often cleaved by PARL into a 53 kD fragment. The protein is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 phosphorylation of mitochondrial proteins can protect against mitochondrial dysfunction due to cellular stresses. PINK1 mutations can cause autosomal recessive early-onset Parkinson disease.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Lin Q, et al. 2020. JCI Insight. :5. PubMed Fiesel FC, et al. 2023. Autophagy. 1711:19. PubMed Yao J, et al. 2022. Autophagy. 18:1879. PubMed Bharath LP, et al. 2020. Cell Metabolism. 32(1):44-55.e6.. PubMed Chung E, et al. 2020. Sci Adv. 6:eaba1193. PubMed
RRID: AB_2783414 (BioLegend Cat. No. 846201) AB_2572127 (BioLegend Cat. No. 846202)
Structure: PINK1 is a 581 amino acid, 63 kD, transmembrane protein that contains an N-terminal mitochondrial localization sequence, a serine/threonine kinase domain, and a C-terminal regulatory domain. PINK1 is proteolytically cleaved by PARL into a 55 kD fragment.
Distribution: Mitochondria and cytoplasm.
Function: PINK1 is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of Parkin protein (PARK2). PARL-cleaved PINK1 is a cytoplasmic probe for damaged mitochondria. PINK1 phosphorylation of mitochondrial proteins can protect against mitochondrial dysfunction due to cellular stresses.
Interaction: PARKIN, Beclin 1, FBX07, DJ-1, HSP90/CDC37.
Ligand/Receptor: PARKIN, Beclin 1, FBX07, DJ-1, HSP90/CDC37.
Biology Area: Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family: Mitochondrial Markers
Antigen References: 1. Trempe JF, Fon EA. 2013. Front Neurol. 4:38. 2. Durcan TM, Fon EA. 2015. Genes Dev. 29(10):989.
Gene ID: 65018
UniProt: View information about PINK1 on UniProt.org
Clone: DU46-1.1
Regulatory Status: RUO
Other Names: PTEN induced putative kinase 1, BRPK, PARK6, Serine/threonine-protein kinase PINK1
Isotype: Mouse IgG1, κ