Biolegend, 848301, Purified anti-α-Synuclein, 80-96 Antibody, 25μg

Alpha-synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of α-synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms include, α-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH-Conjugated synthetic peptide that included amino acid residues 80-96 of human α-synuclein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested Direct ELISA, WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 10 µg/ml is suggested. For Direct ELISA, the suggested use of this reagent is 0.02 - 3.0 per ml. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not cross-react with ß or ?-synucleins using ELISA and WB as detection method.
Product Citations: Gustavsson N, et al. 2021. Acta Neuropathol Commun. 9:153. PubMed
RRID: AB_2650687 (BioLegend Cat. No. 848301) AB_2650688 (BioLegend Cat. No. 848302)
Structure: α-synuclein's canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
Distribution: Tissue distribution: Primarily nervous system, but lower expression in other tissues such as blood and skin. Cellular distribution: Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: α-Synuclein, Phospho-Proteins
Antigen References: 1. Mor DE, et al. 2016. Neurobiol Dis. 88:66. PubMed 2. Jucker M & Walker LC. 2013. Nature. 501(7465):45. PubMed 3. Bartels T, et al. 2011. Nature. 477(7362): 107. PubMed 4. Devine MJ, et al. 2011. Mov Disord. 26:2160. PubMed
Gene ID: 6622
UniProt: View information about alpha-Synuclein 80-96 on UniProt.org
Clone: A15115A
Regulatory Status: RUO
Other Names: Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP), PARK1, NACP, PD1
Isotype: Mouse IgG1, κ