Biolegend, 848401, Purified anti-α-Synuclein (C-Terminal Truncated x-122) Antibody, 25μg

Alpha-synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of α-synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms include, α-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy. Phosphorylation of α-synuclein at Tyr39 may modulate clearance of the protein and contribute to Parkinson's disease.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH-conjugated synthetic peptide of α-synuclein with C-terminus terminating at Asn122.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality testedDirect ELISA - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 10 µg/ml is suggested. For Direct ELISA, a concentration range of 0.02 - 3.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2650689 (BioLegend Cat. No. 848401) AB_2650690 (BioLegend Cat. No. 848402)
Structure: α-synuclein's canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
Distribution: Tissue sources: Primarily nervous system, but lower expression in other tissues. Distribution: Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: α-Synuclein
Antigen References: 1. Mor DE, et al. 2016. Neurobiol Dis. 88:66. PubMed 2. Jucker M & Walker LC. 2013. Nature. 501(7465):45. PubMed 3. Bartels T, et al. 2011. Nature. 477(7362): 107. PubMed 4. Devine MJ, et al. 2011. Mov Disord. 26:2160. PubMed 5. Mishizen-Eberz AJ et al. 2003. J Neurochem. 86:836. PubMed 6. Games D et al. 2014. J Neurosci. 34(28):9441. PubMed
Gene ID: 6622
UniProt: View information about alpha-Synuclein on UniProt.org
Clone: A15127A
Regulatory Status: RUO
Other Names: Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP), PARK1, NACP, PD1
Isotype: Mouse IgG1, κ