Biolegend, 849502, Purified anti-Tau Phospho (Ser262) Antibody, 100μg

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain.  Tau isoforms are differentially expressed during development.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Tau Phospho (Ser262) peptide conjugated to KLH.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested Direct ELISA - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For Direct ELISA, the suggested use of this reagent is 0.05 - 1.0 per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2687294 (BioLegend Cat. No. 849501) AB_2687295 (BioLegend Cat. No. 849502)
Structure: Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution: Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle. Cellular distribution: cytoskeleton, nucleus, plasma membrane, and cytosol.
Function: Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction: Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: Phospho-Proteins
Antigen References: 1. Augustinack JC, et al. 2002. Acta Neuropath. 103(1):26-35. PubMed. 2. Seubert P, et al. 1995. J Biol Chem 270(32):18917-22. PubMed. 3. Dong Y, et al. 2012. PLoS One 7(6):e39386. PubMed.
Gene ID: 4137
UniProt: View information about Tau Phospho Ser262 on UniProt.org
Clone: A15091A
Regulatory Status: RUO
Other Names: Microtubule associated protein tau
Isotype: Mouse IgG2b, κ