Biolegend, 850202, Purified anti-β-Dystroglycan Antibody, 100μg

Dystroglycan is a central component of dystrophin-glycoprotein complex that is encoded by the DAG1 gene. Dystroglycan links the cytoskeletal structure to the extracellular matrix. Dystroglycan is proteolytically cleaved to 2 functional peptides, α- and β-dystroglycan. β-dystroglycan is a transmembrane protein, which acts as a cell adhesion receptor in human tissues. The interactions between β-dystroglycan and dystrophin and Utrophin scaffolds axin to the cytoskeleton. β-dystroglycan also has been reported to play a role in cell adhesion-mediated signaling and is implicated in cell polarity.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH-conjugated peptide corresponding to the last 15 amino acids (KNMTPYRSPPPYVPP) of L-Dystroglycan.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified IHC-F, ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 0.1 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Praissman JL, et al. 2016. Elife. E14473 (ICC) Vasquez-Limeta A, et al. 2014. PLoS One. 9(3):e90629 (WB) Martinez-Vieyra IA, et al. 2013. biochim Biophys Acta. 1833(3):698 (ICC, WB) Beedle AM, et al. 2012. J Clin Invest. 122(9):3330 (IHC-F, WB) Hu H, et al. 2011. Neurosci Lett. 489(1): 10 (WB)
Product Citations: Dai J, et al. 2022. Nat Cancer. 3:25. PubMed
RRID: AB_2715870 (BioLegend Cat. No. 850201) AB_2715871 (BioLegend Cat. No. 850202)
Structure: Dystroglycan is an 895 amino acid protein with a molecular mass of ~97 kD. β-dystroglycan, a cleavage product of dystroglycan, is a 242 amino acid protein with a molecular mass of ~43 kD.
Distribution: Tissue distribution: Primarily expressed in muscle tissue. Also present in other tissue including CNS. Cellular distribution: nucleus, plasma membrane, and cytoskeleton.
Function: β-dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.
Interaction: Interacts with Dystrophin, Utrophin and other cytoskeletal components such as axin.
Ligand/Receptor: Receptor for Dystrophin and Utrophin
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Antigen References: 1. Murphy S, et al. 2015. Comput Struct Biotechnol J. 14:20-7 2. Moore CJ, et al. 2012. Neuromuscul Disord. 22(11): 959
Gene ID: 1605
UniProt: View information about beta-Dystroglycan on UniProt.org
Clone: 7D11
Regulatory Status: RUO
Other Names: Beta-dystroglycan, Beta-DG
Isotype: Mouse IgG1, κ