Biolegend, 850502, Purified anti-TFAM Antibody, 100μg

TFAM (Transcription Factor A, Mitochondrial) binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation, and DNA replication and repair. The protein is required for accurate and efficient promoter recognition by mitochondrial RNA polymerase. Sequence polymorphisms in the TFAM gene are associated with Alzheimer's and Parkinson's diseases. Other diseases associated with TFAM include Mitochondrial DNA Depletion Syndrome, Hepatocerebral Form and Deoxyguanosine Kinase Deficiency.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full-length human TFAM protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody recognizes endogenous TFAM in human brain tissues in WB and IHC applications but does not recognize mouse TFAM.
Application References(PubMed link indicates BioLegend citation): Wang YE, et al. 2013. PLoS One 8(8):e74513. (WB) Caielli S, et al. 2016. J Exp Med. 213(5):697-713. (WB)
Product Citations: Fihurka O, et al. 2023. Biomolecules. 13:. PubMed von Walden F, et al. 2021. Dev Med Child Neurol. 63:1204. PubMed Fihurka O, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID: AB_2716009 (BioLegend Cat. No. 850501) AB_2716010 (BioLegend Cat. No. 850502)
Structure: TFAM is synthesized as a precursor form with a mitochondrial targeting peptide, which is removed by processing after import to the mitochondria. Mature TFAM has an apparent molecular weight of ~25 kD. Alternative splicing results in multiple transcript variants.
Distribution: Tissue distribution: Central nervous system, skin, kidney, lung, thyroid, lymph nodes, breast, esophagus, and heart. Cellular distribution: Mitochondrion, nucleus, and cytosol.
Function: TFAM functions in mitochondrial transcription regulation, and DNA replication and repair.
Interaction: TFB1M, TFB2M, CLPX
Biology Area: Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family: Mitochondrial Markers
Antigen References: 1. Bestwick ML, Shadel GS. 2013. Trends Biochem Sci 38(6):283-91. 2. Ramachandran A, et al. 2017. Nucleic Acids Res 45(2):861-874. 3. Günther C, et al. 2004. Neurosci. Lett. 369: 219–223.
Gene ID: 7019
UniProt: View information about TFAM on UniProt.org
Clone: 18G102B2E11
Regulatory Status: RUO
Other Names: Transcription factor A, mitochondrial, mtTFA, MtTF1, TCF6, TCF-6, Transcription Factor 6
Isotype: Mouse IgG2b, κ