Biolegend, 851502, Purified anti-DJ-1 (PARK7) Antibody, 100μg

DJ-1 (PARK7) belongs to the peptidase C56 family of proteins and is expressed in almost all human cells and tissues. DJ-1 is a multi-functional protein associated with mitochondrial function, mitophagy, and male fertility. It acts as an oxidative stress sensor, redox-sensitive chaperone and protease. Therefore, the protein has an important role in cell protection against oxidative stress and cell death. DJ-1 is also associated with prostate cancer, and specific mutations of the protein are linked to autosomal recessive, early onset Parkinson’s disease.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human DJ-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedDirect ELISA, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For Direct ELISA, a concentration range of 0.002 - 1.0 µg/mL is recommended. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2721732 (BioLegend Cat. No. 851501) AB_2721733 (BioLegend Cat. No. 851502)
Structure: Human DJ-1 is a 189 amino acid protein with an apparent molecular weight of 20 kD. The protein consists of 7 β-strands and 7 α-helices, and exits as a dimer.
Distribution: Tissue distribution: Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in brain and placenta. Cellular distribution: Mitochondria, nucleus, cytosol, plasma membrane, and extracellular.
Function: DJ-1 functions as an oxidative stress sensor and redox-sensitive chaperone.
Interaction: PINK1, Parkin, EFCAB6/DJBP, OTUD7B, BBS1, HIPK1, CLCF1 and MTERF.
Biology Area: Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Molecular Family: Mitochondrial Markers
Antigen References: 1. Saito Y. 2014. J. Clin. Biochem. Nutr. 54(3):138-44. PubMed 2. Ariga H, et al. 2013. Oxid. Med. Cell Longev. 2013:683920. PubMed 3. Bonifati V, et al. 2003. Science (5604): 256–9. PubMed
Gene ID: 11315
UniProt: View information about DJ-1 on UniProt.org
Clone: A16125E
Regulatory Status: RUO
Other Names: Protein DJ-1, oncogene, DJ1, PARK7, Parkinson disease protein 7, Parkinsonism-Associated Deglycase, HEL-S-67p, epididymis secretory sperm binding protein Li 67p, GATD2, Protein Deglycase DJ-1
Isotype: Mouse IgG2b, κ