Biolegend, 851702, Purified anti-MAG Antibody, 100μg

Myelin-associated glycoprotein (MAG) is a cell membrane glycoprotein and a member of the siglec (sialic acid-binding immunoglobulin-like lectins) family. MAG has two isoforms in myelinated structures in the nervous system. MAG is expressed on oligodendrocytes and Schwann cells and is believed to be involved in myelination during nerve regeneration. MAG knockout mice develop quite severe pathological abnormalities associated with myelin instability.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified MAG protein isolated from human central nervous system.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Dobersen MJ, et al. 1985. Neurochem Research. 4: 499 (WB and IHC) Cao Z, et al. 2007. J Neurosci. 34: 9146 (WB)
Product Citations: Yang L, et al. 2024. Sci Adv. eadj2445:10. PubMed
RRID: AB_2749879 (BioLegend Cat. No. 851701) AB_2749880 (BioLegend Cat. No. 851702)
Structure: MAG is a 626 amino acid protein with a molecular mass of 70 kD.
Distribution: Tissue distribution: Central and peripheral nervous system, in periaxonal myelin and at Schmidt-Lanterman incisures. Cellular distribution: Plasma membrane
Function: MAG is involved in the process of axon myelination and maintenance of myelin sheath along axons. MAG also functions as a receptor that promotes differentiation, maintenance and survival of oligodendrocytes. Aberrant immunoreactivity to MAG has been suggested to play a role in the pathogenesis of multiple sclerosis.
Interaction: MAG binds to neuronal sialic acid-containing gangliosides and to glycoproteins RTN4R and RTN4RL2.
Biology Area: Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family: Siglec Molecules
Antigen References: Dobersen MJ, et al. 1985. Neurochem Research. 4: 499 McKerracher L and Rosen KM. 2015. Front Mol Neurosci. 8: 51 Vallat JM, et al. 2016. Expert Rev Neurother. 9: 1111
Gene ID: 4099
UniProt: View information about MAG on UniProt.org
Clone: B11F7
Regulatory Status: RUO
Other Names: Siglec-4a, Myelin-associated Glycoprotein,
Isotype: Mouse IgG2b, κ