Biolegend, 852001, Purified anti-TFEB Antibody, 25μg

The autophagy-lysosomal pathway is involved in the degradation of long-lived proteins. The transcription factor EB (TFEB) plays a critical role in regulating basic cellular processes, such as lysosomal biogenesis and autophagy. The subcellular localization and activity of TFEB are regulated by mechanistic target of rapamycin (mTOR)-mediated phosphorylation. TFEB is also involved in physiological processes, and it induces the intracellular clearance of pathogenic factors in a variety of diseases, such as Parkinson's and Alzheimer's, suggesting that novel therapeutic strategies could be based on the modulation of TFEB activity.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2728585 (BioLegend Cat. No. 852001) AB_2728586 (BioLegend Cat. No. 852002)
Structure: TFEB is a 476 amino acid protein with a molecular weight mass of approximately 53kD.
Distribution: Tissue distribution: TFEB is expressed in numerous cell types of the central nervous system (CNS). Cellular distribution: Cytosolic and nucleus.
Function: TFEB is a major regulator of autophagy and lysosomal biogenesis.
Interaction: TFEB interacts with the mTOR complex and its activity is thought to be regulated through the phosphorylation by mTOR.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Martini-Stoica H, et al. 2016. Trends Neurosci. 221-234. Napolitano G, Ballabio A. 2016. J. Cell Sci. 129: 2475-81. Raben N, Puertollano R. 2016. Annu. Rev. Cell Dev. Biol. 32: 255-278.
Gene ID: 7942
UniProt: View information about TFEB on UniProt.org
Clone: A17106C
Regulatory Status: RUO
Other Names: Transcription factor EB
Isotype: Mouse IgG2b, κ