Biolegend, 852102, Purified anti-Sortilin Antibody, 100μg

Sortilin was originally purified from human brain tissue and was identified as a new lipoprotein receptor, which was later identified as a multifunctional sorting receptor of the VPS10P-domain receptor family. Although Sortilin is predominately expressed in neurons of the central and the peripheral nervous systems, it is also expressed in metabolic tissues including liver. Sortilin functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Sortilin is critical for lipid metabolism, and it has been implicated in lipid disorders. Sortilin is also an APOE receptor implicated in Alzheimer disease.
100μg
Verified Reactivity: Human, Rat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human SORT1 recombinant protein (327-638 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry, a concentration range of 2.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2728587 (BioLegend Cat. No. 852101) AB_2728588 (BioLegend Cat. No. 852102)
Structure: Sortilin is an 831 amino acid protein with a molecular mass of 95 kD.
Distribution: Tissue distribution: Sortilin is expressed in numerous tissues including brain, spinal cord, heart, skeletal muscle, thyroid, placenta, and testis. Cellular distribution: Plamsa membrane, cytosol, endosome, endoplasmic reticulum, lysosome, and golgi apparatus.
Function: Sortilin functions as a cell surface receptor and mediates intracellular trafficking of diverse endogenous or exogenous protein substrates between the trans-Golgi network and plasma membrane compartments.
Interaction: Sortilin interacts with p75 Neurotrophin Receptor and is localized together with the golgi marker GM130 and the early endosome marker EEA1.
Ligand/Receptor: Sortilin functions as lipoprotein receptor for neurotrophins and other growth factors.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Transcription Factors
Molecular Family: Golgi Markers
Antigen References: Skeldal S, et al. 2012. J. Biol. Chem. 287:43798-809. Herda S, et al. 2012. Immunity. 37:854-66. Gao A, et al. 2017. DNA Cell Biol. 36:1050-1061. Carlo AS. 2013. Prion. 7:378-82.
Gene ID: 6272
UniProt: View information about Sortilin on UniProt.org
Clone: W16078A
Regulatory Status: RUO
Other Names: 100 kD NT receptor
Isotype: Rat IgG2a, κ