Biolegend, 852302, Purified anti-Nicastrin Antibody, 100μg

Nicastrin is one of the essential components of the γ-secretase complex. The γ-secretase complex is one of the proteases involved in processing amyloid precursor protein (APP) to amyloid beta (Aβ). Nicastrin is not catalytically active, instead, it promotes the maturation and localization of other catalytic and regulatory units of the γ-secretase complex. Nicastrin has alsp been reported to regulate neprilysin, which is the enzyme involved in the degradation of Aβ fragment.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to the C-terminal 18 a.a. of mouse Nicastrin.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: In human, the antibody also detects the deglycosylated nicastrin, which is observed at ~ 78kD.
Application References(PubMed link indicates BioLegend citation): Esselens C, et al. 2004. J. Cell Biol. 166(7):1041. (WB)
Product Citations: Aron L, et al. 2023. Nat Commun. 7030:14. PubMed
RRID: AB_2728591 (BioLegend Cat. No. 852301) AB_2728592 (BioLegend Cat. No. 852302)
Structure: Deglycosylated nicastrin is a 709 amino acid protein with a molecular mass of ~78 kD. Higher molecular weight band of ~110 kD corresponding to glycosylated nicastrin is observed by WB.
Distribution: Tissue Distribution: Ubiquitously expressed. Cellular: Distribution: Plasma Membrane, endoplasmic reticulum, lysosome, golgi apparatus, and cytoskeleton.
Function: Nicastrin is an essential component of the γ-secretase complex that processes amyloid precursor protein to amyloid beta. It is also involved in degradation of amyloid beta fragments.
Interaction: Presenilin, APH-1, PEN-2
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Molecular Family: Enzymes and Regulators, Secretases
Antigen References: Yang D, et al. 2002. J. Biol. Chem. 31:28135. Johnson DS, et al. 2017. Cold Spring Harb Perspect Med. 10.1101/cshperspect.a024067. Zhang X, et al. 2014. Front Cell Neurosci. 10.3389/fncel.2014.00427.
Gene ID: 23385
UniProt: View information about Nicastrin on UniProt.org
Clone: 9C3
Regulatory Status: RUO
Other Names: NCSTN
Isotype: Mouse IgG2b, κ