Biolegend, 853202, Purified anti-ATF6β Antibody, 100μg

ATF6 encodes a transcription factor that is anchored in the endoplasmic reticulum (ER) and activated during the unfolded protein response (UPR) to protect cells from ER stress. Under conditions of ER stress, ATF6 is transported from the ER to the Golgi apparatus, where it is cleaved by Golgi-resident proteases to release the cytosolic DNA-binding portion. Then the processed ATF6 moves to the nucleus to activate gene expression. Deletion of the isoform activating transcription factor 6α (ATF6α) and its paralog ATF6β results in embryonic lethality and notochord dysgenesis in nonhuman vertebrates, and loss-of-function mutations in ATF6α are associated with malformed neuroretina and congenital vision loss in humans. Altered ATF6 function in neurodegenerative disorders such as  Parkinson's disease has been observed. 100μg Verified Reactivity: Human Antibody Type: Monoclonal Host Species: Rat Immunogen: Human ATF6β recombinant protein (1-250 a.a.) expressed in E. coli. Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation: The antibody was purified by affinity chromatography. Concentration: 0.5 mg/ml Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Application: WB - Quality tested ICC - Verified Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. RRID: AB_2728607 (BioLegend Cat. No. 853201) AB_2728608 (BioLegend Cat. No. 853202) Structure: ATF6β is a 703 amino acid protein with a molecular weight mass of ~80 kD. Distribution: Tissue distribution: ATF6β is ubiquitously expressed in numerous cell types including cells in of the central nervous system (CNS). Cellular distribution: Cytoplasmic, nucleus, ER, and golgi apparatus. Function: ATF6β is a transmembrane glycoprotein that functions as a transcription activator and initiates the unfolded protein response during ER stress. Interaction: ATF6β interacts with ER chaperone immunoglobulin-binding protein (BiP), and can be cleaved by lumenal site 1 protease (S1P) and intra-membrane site 2 protease (S2P). Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Transcription Factors Molecular Family: Endoplasmic Reticulum Markers Antigen References: Shen J, et al. 2002. Dev Cell. 3:99-111 Ron D and Walter P. 2007. Nat Rev Mol Cell Biol. 8: 519-529 Credle JJ, et al. 2015. Neurobiol Dis. 76: 112-125 Jin JK, et al. 2017. Circ Res. 120: 862-875 Kroeger H, et al. 2018. Sci Signal. 11: 5785 Gene ID: 1388 UniProt: View information about ATF6beta on UniProt.org Clone: W17035A Regulatory Status: RUO Other Names: Cyclic AMP-dependent transcription factor ATF-6 beta, ATF-6 beta, Activating transcription factor 6 beta, ATF6-beta Isotype: Rat IgG2b, κ