Biolegend, 853314, Alexa Fluor® 647 anti-TMEM119 (Extracellular) Antibody, 100μg

Microglia are the resident parenchymal myeloid cells of the CNS, and play an important role in development, homeostasis, disease, and injury. TMEM119 is a transmembrane protein and was identified as a microglia-specific marker in both mouse and human CNS. Moreover, TMEM119 immunoreactivity has been exclusively detected on a subset of Iba1(+) CD68(+) microglia with ramified and amoeboid morphologies in the brains of neurodegenerative diseases. TMEM119 also plays an important role in bone formation and normal bone mineralization. It promotes the differentiation of myoblasts into osteoblasts. and it may induce the commitment and differentiation of myoblasts into osteoblasts. TMEM119 is also essential for normal spermatogenesis and late testicular differentiation.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human TMEM119 protein (extracellular domain)
Formulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azideTest size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: µg size: 0.5 mg/mLLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC, IHC-P - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis and formalin-fixed paraffin-embedded immunohistochemical staining. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 2.5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: For microscopy, the antibody was found to work best when using fresh frozen human tissues. Communication by Dr. Frederick Christian Bennett at Stanford University, under review in Neuron. *The human TMEM119 full length plasmid was a courtesy gift from Dr. Ben Barres at Stanford University.
Additional Product Notes: For this antibody, we conveniently offer both microgram amounts and test sizes. Test sizes are available for convenience when using this antibody for flow cytometry/intracellular flow cytometry.
Application References(PubMed link indicates BioLegend citation): Bennett FC, et al. 2018. Neuron. 98:1-14 PubMed
RRID: AB_3097501 (BioLegend Cat. No. 853311) AB_3097501 (BioLegend Cat. No. 853312) AB_3097501 (BioLegend Cat. No. 853314)
Structure: TMEM119 is an 283 amino acid protein with a molecular mass of 30 kD.
Distribution: Tissue distribution: TMEM119 is expressed in the brain, bone, and lymphoid tissues. Cellular distribution: Membrane and endoplasmic reticulum.
Function: TMEM119 is an important microglia marker and is implicated in neurodegenerative diseases.
Interaction: TMEM119 interacts with SMAD1, SMAD5 and RUNX2.
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Antigen References: Bennett ML, et al. 2016. Proc Natl Acad Sci U S A. 113 Satoh J, et al. 2016. Neuropathology. 36:39-49 Kawao N and Kaji H. 2015. J Cell Biochem. 116:687 Zrzavy T, et al. 2017. Brain. 140:1900-1913
Gene ID: 338773
UniProt: View information about TMEM119 on UniProt.org
Clone: A16075D
Regulatory Status: RUO
Other Names: Transmembrane protein 119
Isotype: Mouse IgG2b, κ