Biolegend, 853502, Purified anti-FBXO7 Antibody, 100μg

F-box only protein 7 (FBXO7) is involved in the ubiquitination process during protein degradation. FBXO7 is a subunit of the SCF (SKP1/cullin‐1/F‐box protein) complex. The scaffold proteins cullin‐1 and SKP1, the RING subunit Rbx1 together with the substrate‐recruiting subunit FBXO7 form a functional E3 ubiquitin ligase. FBXO7 is implicated in Parkinson’s disease. In addition to reduced proteasome activity, loss of FBXO7 in mice leads to Parkinsonism-like phenotype. Human FBXO7 is mainly localized to the nucleus, while the mutation observed in patients with parkinsonian-pyramidal syndrome leads to impaired nuclear localization of this protein.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human FBXO7 recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is specific for human FBXO7 and does not cross-react with rat or mouse FBXO7, verified by western blot. The two bands observed in western blot potentially indicate the two isoforms of FBXO7.
RRID: AB_2734650 (BioLegend Cat. No. 853501) AB_2734651 (BioLegend Cat. No. 853502)
Structure: FBXO7 is a 522 amino acid protein with a molecular mass of ~60 kD.
Distribution: Tissue distribution: FBXO7 is ubiquitously expressed in the many tissues including brain and muscle. Cellular distribution: Cytoplasmic and nucleus.
Function: FBXO7 is involved in the ubiquitin-proteasome system, and defects in FBXO7 cause Parkinson's disease-like phenotypes.
Interaction: FBXO7 interacts with the members of the SCF (SKP1/cullin‐1/F‐box protein) complex.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family: Nuclear Markers
Antigen References: Vingill S, et al. 2016. EMBO J. 35: 2008-25. Zhao T, et al. 2011. PLoS One. 6:e16983. Zhou ZD, et al. 2015. Hum. Mol. Genet. 24:6314-30.
Gene ID: 25793
UniProt: View information about FBXO7 on UniProt.org
Clone: W16207A
Regulatory Status: RUO
Other Names: F-box only protein 7
Isotype: Rat IgG2a, κ