Biolegend, 854001, Purified anti-Testosterone Antibody, 25μg

In vertebrate, Testosterone is the major male sex hormone that is essential to the development of reproductive organs and secondary sexual characteristics. It also plays a key role in muscle development, body mass increase and body fair growth. Importantly, it is critical to sperm development. In vivo, testosterone is primarily secreted by testicles (in male), and by ovaries (in female), and eventually metabolized in liver. In the central nervous system, Testosterone is one of the key components of the hypothalamic-pituitary-adrenal axis, which is activated acutely by dominance challenger. It has been reported that different level of sex hormones, including testosterone, determines human behavior, as well as brain development and recovery after injury.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Testosterone-3-(O-carboxymethyl)oxime coupled to bovine serum albumin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested Direct ELISA - Verified
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 10 - 150 ng/mL is recommended. For Direct ELISA applications, a concentration range of 1.0 - 10 ng/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody has been tested against human and mouse sera.
Application References(PubMed link indicates BioLegend citation): Fantl VE, et al. 1983. J Steroid Biochem. 19(5):1605 (PubMed)
RRID: AB_2750291 (BioLegend Cat. No. 854001) AB_2750292 (BioLegend Cat. No. 854002)
Structure: Testosterone is an Androgen with a molecular mass of 288.431 g/mol.
Distribution: Tissue Source: Primarily in reproductive organs (Testis, ovaries), trace amount in other organs including brain and liver. Cellular Source: Secreted molecule.
Ligand/Receptor: Binds to androgen receptors.
Biology Area: Cell Biology, Neuroscience, Synaptic Biology
Antigen References: Dong DX & Ji ZG. 2017. Chin Med J (Engl). 130:2991 Roselli CE. 2017. J Neuroendocrinol. Doi:10.1111/jne.12562 Mohammad OS, et al. 2017. Cancers (Basel). 9(12).pii:E166
Gene ID: NA
UniProt: View information about Testosterone on UniProt.org
Clone: 3T16
Regulatory Status: RUO
Other Names: Testosterone
Isotype: Mouse IgG2b, κ