Biolegend, 855001, Purified anti-FUS Antibody, 25μg

FUS/TLS is an RNA-binding protein that was originally discovered as a fusion protein caused by chromosomal translocation in cancer. FUS/TLS is as an important member of the cellular DNA repair response. The C-terminal end of FUS is involved in protein and RNA binding, while the N-terminal motif of FUS plays a role in transcriptional activation. FUS was also shown to accumulate in the cytoplasm of neurons under hyperosmotic conditions, and FUS mislocalization has been implicated as being the first step toward disease.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human TLS/FUS recombinant protein fused with GST tag.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2750473 (BioLegend Cat. No. 855001) AB_2750474 (BioLegend Cat. No. 855002)
Structure: FUS is a 526 amino acid protein with an apparent molecular mass of ~ 53 kD.
Distribution: Tissue Distribution: Ubiquitously expressed Cellular Distribution: Nucleus and cytosol
Function: FUS/TLS binds to DNA and maintains genomic integrity.
Interaction: Interacts with HDAC1, RNA Polymerase II, FUSIP1, ILF3, PRMT1, RELA, SPI1, and TNPO1.
Cell Type: Astrocytes, Neurons, Oligodendrocytes
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Synaptic Biology
Molecular Family: Postsynaptic proteins, Presynaptic proteins
Antigen References: Efimova AD, et al. 2017. Mol Biol (Mosk) 51:387. Ederle H, Dormann D. 2017. FEBS Lett. 591:1489. Guerrero EN, et al. 2016. Prog. Neurobiol. 145-146:78.
Gene ID: 2521233908217385
UniProt: View information about FUS on UniProt.org
Clone: 10F7
Regulatory Status: RUO
Other Names: FUS, TLS
Isotype: Mouse IgG1, κ