Biolegend, 858601, Purified anti-CD133 (Prominin-1) Antibody, 25μg

Prominin-1 (Prom1), also known as CD133, is a transmembrane glycoprotein located mainly on cellular protrusions of neurons and glial stem cells in the nervous system. CD133 level has been used as marker for isolation of cancer stem cells from various gliomas. Prom1 is also expressed in other tissues including kidney, mammary glands, and uterus.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2801221 (BioLegend Cat. No. 858601) AB_2801222 (BioLegend Cat. No. 858602)
Structure: Prom1 is an 865 amino acid protein with an apparent molecular mass of ~ 97 kD.
Distribution: Cellular Distribution: Plasma membrane, extracellular, and endoplasmic reticulum. Tissue Distribution: Glioblastomas; liver,gallbladder, cervix, uterus, and endometrium.
Function: Used as a marker for isolation of cancer stem cells.
Interaction: CDHR1, Actin, NAT8, NAT8B
Cell Type: Neural Stem Cells, Neurons
Biology Area: Immuno-Oncology, Neuroscience
Molecular Family: CD Molecules
Antigen References: Nagare RP, et al. 2017. Curr Stem Cell Res Ther. 12(1):37 Dahlrot RH, et al. 2013. Int J Clin Exp Pathol. 6(3):334 Donovan LK, et al. 2012. Cell Prolif. 45(6):527
Gene ID: 8842
UniProt: View information about CD133 on UniProt.org
Clone: 8F2-H6-D6
Regulatory Status: RUO
Other Names: Prom1, Prominin-1, CD133
Isotype: Mouse IgG2a, κ