Biolegend, 862207, Alexa Fluor® 488 anti-Myelin Protein Zero Antibody, 25μg

Myelin protein zero (MPZ), is a membrane glycoprotein mainly expressed in the peripheral nervous system (PNS). It is the major structural component of the myelin sheath, and accounts for at least 50% of all proteins in the PNS. MPZ is expressed by Schewann cells. Mutations in MPZ have been associated with myelin deficiency, which may lead to neuropathies including charcot-Marie-Tooth and Dejerine-Sottas diseases.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Yoshimura K, et al. 1996. Neurosci Res. 25(1):41 (WB, IHC, ELISA)
RRID: AB_2814591 (BioLegend Cat. No. 862207) AB_2814592 (BioLegend Cat. No. 862208)
Structure: MPZ is a 248 amino acid protein with an apparent molecular mass of ~ 28 kD.
Distribution: Tissue Distribution: Peripheral nervous system Cellular Distribution: Plasma membrane, extracellular, endoplasmic reticulum, and lysosome
Function: MPZ is an adhesion molecule involved in the formation of myelin sheath in the peripheral nervous system.
Interaction: Forms homodimer and homotetramer
Biology Area: Neurodegeneration, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules
Antigen References: Chiò A and Lauria G. 2016. Lancet Neurol. 15(1):12 Brennan KM, et al. 2015. Neurosci Lett. 596:14 Tazir M, et al. 2014. J Neurol Sci. 347(1-2):14
Gene ID: 43591752824564
UniProt: View information about Myelin Protein Zero on UniProt.org
Clone: 58A
Regulatory Status: RUO
Other Names: MPZ, Myelin Protein Zero
Isotype: Mouse IgG2b, κ