Biolegend, 862301, Purified anti-Gemin 6 Antibody, 25μg

Gem-associated protein 6 (Gemin 6) is a component of the survival of motor neurons (SMN) macromolecule protein complex, which plays an important role in the assembly and re-structuring of small nucleoproteins and transcriptosomes. The SMN complex localizes to the cytosol and nucleus of eukaryotic cells.  Gemin 6 has been shown to form heterodimers with Gemin 7, and together they bind to unrip and sequester the SMN complex in the cytoplasm for small nuclear ribonucleoproteins (snRNP) assembly 3 .
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is more reactive to mouse compared to human or rat Gemin 6. The recommended usage concentration for Western blotting in mouse is 0.2 - 1 µg/ml, and in human and rat 5.0 - 10 µg/mL
Application References(PubMed link indicates BioLegend citation): Massenet S, et al. 2002. Mol Cell Biol. 22(18):6533 (WB) Battle DJ, et al. 2007. J Biol Chem. 282(38):27953 (IP)
Product Citations: Bizen N, et al. 2022. Cell Death Differ. . PubMed
RRID: AB_2810749 (BioLegend Cat. No. 862301) AB_2810750 (BioLegend Cat. No. 862302)
Structure: Gemin 6 is a 167 amino acid protein with an apparent molecular mass of ~19 kD.
Distribution: Tissue Distribution: Ubiquitously expressed Cellular Distribution: Nucleus and cytosol
Function: Gemin 6 is a component of the SMN complex essential for the formation of snRNP complexes in Eukaryotic cells.
Interaction: Gemin-7, unrip
Cell Type: Mature Neurons, Neurons
Biology Area: Chromatin Remodeling/Epigenetics, Neurodegeneration, Neuroscience
Molecular Family: Nuclear Markers
Antigen References: Lanfranco M, et al. 2017. FEBS Lett. 591(21):3600 Massenet S, et al. 2002. Mol Cell Biol. 22(18):6533 Battle DJ, et al. 2007. J Biol Chem. 282(38):27953
Gene ID: 79833
UniProt: View information about Gemin 6 on UniProt.org
Clone: 6H5/Gemin6
Regulatory Status: RUO
Other Names: Gemin6
Isotype: Mouse IgG1, κ