Biolegend, 862501, Purified anti-HNRNPA1 Antibody, 25μg

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a multi-purpose RNA binding protein involved in RNA metabolism and DNA genomic stability in normal and pathological conditions. hnRNP A1 is ubiquitously expressed and usually associates with nascent RNA polymerase II transcripts. hnRNP A1 is  abundantly expressed in neuronal cells and reproductive tissue. Loss of hnRNP A1 has been observed in several neurodegenerative diseases, including Alzheimer’s disease, multiple sclerosis, amytrophic lateral sclerosis. Structurally, hnRNPA1 has two RNA recognition motifs, which are post-translationally modified to alter its nuclear pore shuttling properties and RNA interaction.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partially purified HeLa HNRNPA1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.1 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2801243 (BioLegend Cat. No. 862501) AB_2801244 (BioLegend Cat. No. 862502)
Structure: hnRNPA1 has two isoforms. Human HNRPNA1 isoform A1-A is a 320 amino acid protein with a molecular mass of 34 kD, and isoform A1-B is a 372 amino acid protein with a molecular mass of 38 kD.
Distribution: Tissue Distribution: Ubiquitous expression Cellular Source: Nucleus and cytoplasm
Function: RNA metabolism, genomic stability
Interaction: Nascent RNA polymerase II transcripts
Cell Type: Neurons
Biology Area: Neurodegeneration, Neuroscience, Protein Synthesis
Antigen References: Bekenstein U, et al. 2013. Mol Cell Neurosci. 56:436-46. Jean-Philippe J, et al. 2014. Biochim Biophys Acta. 1839(4): 251–258.
Gene ID: 31781538229578
UniProt: View information about HNRNPA1 on UniProt.org
Clone: 4B10/HNRNPA1
Regulatory Status: RUO
Other Names: Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1
Isotype: Mouse IgG2a, κ