Biolegend, 862605, PE/Cyanine7 anti-β Catenin 1 Antibody, 25tests

β catenin is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. β catenin also plays a key role in Wnt signaling pathways and thus is involved in neural differentiation, synaptic plasticity, neurodegenerative disease, and prevention of apoptosis. This protein also binds to adenomatous polyposis coli, which is mutated in the adenomatous polyposis of the colon.
25tests
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Guo J, et al. 2018. Cell Res. 28:1141 (IHC-P) Kisoh K, et al. 2017. Mol Neurobiol. 54:7917 (WB) Uribe RA, et al. 2015. J Cell Biol. 4:815 (IHC-F)
RRID: AB_2927830 (BioLegend Cat. No. 862605) AB_2927830 (BioLegend Cat. No. 862606)
Structure: β Catenin is a 781 amino acid protein with an apparent molecular mass of ~ 86 kD.
Distribution: Tissue Distribution: Ubiquitously expressed Cellular Distribution: Plasma membrane, cytoskeleton, nucleus, cytosol, and extracellular
Function: β Catenin is a dual functional protein involved in cell-cell adhesion and gene transcription.
Interaction: E-cadherin/CDH1, LEF1, TCF4, etc.
Antigen References: Dias C, et al. 2014. Nature. 516(7529): 51 Rosiles A, et al. 2016. CNS Neurol Disord Drug Targets. 15(1):3 Na K, et al. 2017. Anticancer Res. 37(6):3249 Zhu P, et al. 2018. Cancer Res Treat. 50(4):1452
Gene ID: 14991238784353
UniProt: View information about beta Catenin 1 on UniProt.org
Clone: 15B8
Regulatory Status: RUO
Other Names: β-catenin, CTNNB1
Isotype: Mouse IgG1, κ