Biolegend, 862802, Purified anti-SMN (Gemin 1) Antibody, 100μg

SMN1/Gem-associated protein 1 (Gemin 1) is a component of the survival of motor neurons (SMN) macromolecule protein complex, which plays an important role in the assembly and re-structuring of small nucleoproteins and transcriptosomes. The SMN complex localizes to the cytosol and nucleus of eukaryotic cells. Mutations of SMN1 lead to spinal muscular atrophy.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC, IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Kobayashi M, et al. 2017. Disease Models & Mechanisms. (10):215 (IHC-F) Spiro Z, et al. 2016. Scientific Reports. (6):27470 (ICC) Yong J, et al. 2004. Molecular and Cellular Biology. 24(7):2747 (WB)
RRID: AB_2810753 (BioLegend Cat. No. 862801) AB_2810754 (BioLegend Cat. No. 862802)
Structure: SMN1 is a 294 amino acid protein with an apparent molecular mass of ~ 32 kD.
Distribution: Tissue Distribution: Brain, pancreas, gallbladder, bladder, testis, lymph node, and bone marrow Cellular Distribution: Nucleus and cytosol
Function: SMN1 is a component of the SMN complex essential for the formation of snRNP complexes in Eukaryotic cells.
Interaction: SMN1 forms homodimers or found in complex with other SMN/GEMIN proteins.
Cell Type: Neurons
Biology Area: Chromatin Remodeling/Epigenetics, Neurodegeneration, Neuroscience, Synaptic Biology, Transcription Factors
Antigen References: Maretina MA, et al. 2018. Curr Genomics. 19(5):339 Singh RN, et al. 2018. Adv Neurobiol. 20:31 Groen EJN, et al. 2018. Nat Rev Neurol. 14(4):214
Gene ID: 66066607
UniProt: View information about SMN on UniProt.org
Clone: 2B1
Regulatory Status: RUO
Other Names: SMN1, SMN, SMN2, SMNT, GEMIN1
Isotype: Mouse IgG1, κ