Biolegend, 862901, Purified anti-Gemin 2 Antibody, 25μg

Gemin 2 is a component of the survival of motor neurons (SMN) macromolecule protein complex, which plays an important role in the assembly and re-structuring of small nucleoproteins and transcriptosomes. The SMN complex localizes to the cytosol and nucleus of eukaryotic cells. Gemin 2 is the only gemin protein that has no homology to any other gemins. Recent studies have suggested that the role of Gemin 2 is to organize 5 Sm proteins into a partial ring for snRNA binding.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Battle D, et al. 2007. J Biol Chem. 282(38):27953 (WB, ICC)
Product Citations: Bizen N, et al. 2022. Cell Death Differ. . PubMed
RRID: AB_2810755 (BioLegend Cat. No. 862901) AB_2810756 (BioLegend Cat. No. 862902)
Structure: Gemin 2 is a 280 amino acid protein with an apparent molecular mass of ~ 32 kD.
Distribution: Tissue Distribution: Brain, skeletal muscle, gallbladder, and testis Cellular Distribution: Nuclear and cytosol
Function: Gemin 2 is a component of the SMN complex that is essential for the formation of snRNP complexes. It organizes 5 Sm proteins into a partial ring for snRNA binding.
Interaction: Gemin 2 binds to SM proteins and is found in complex with other SMN/GEMIN proteins.
Cell Type: Neurons
Biology Area: Chromatin Remodeling/Epigenetics, Neurodegeneration, Neuroscience, Synaptic Biology, Transcription Factors
Antigen References: Battle D, et al. 2007. J Biol Chem. 282(38):27953 Gupta K, et al. 2015. J Biol Chem. 290(33):20185
Gene ID: 84876660384404
UniProt: View information about Gemin 2 on UniProt.org
Clone: 2S7
Regulatory Status: RUO
Other Names: GEMIN 2, SIP1
Isotype: Mouse IgG1, κ