Biolegend, 864908, Alexa Fluor® 594 anti-α-Synuclein, aggregated Antibody, 100μg

α-synuclein is expressed principally in the nervous system, but it is also produced in the other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in the presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in the nuclei and mitochondria. At least three isoforms of synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms are α-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-Synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human alpha-synuclein aggregates
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
RRID: AB_2861089 (BioLegend Cat. No. 864907)
Structure: Human α-synuclein is a 140 amino acid protein with a molecular mass of 14 kD.
Distribution: Tissue distribution: Primarily nervous system, but lower expression in other tissues such as blood and skin. Cellular distribution: Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.
Function: α-synuclein plays several roles in synaptic activity such as synaptic vesicle trafficking and neurotransmitter release. It is also involved in the regulation of gene expression.
Interaction: Interacts with various proteins, such as UCHL1, phospholipase D,histones and SNARE components.
Cell Type: Neurons
Biology Area: Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Synaptic Biology
Molecular Family: α-Synuclein, Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Gene ID: 6622
UniProt: View information about alpha-Synuclein aggregated on UniProt.org
Clone: A17183A
Regulatory Status: RUO
Other Names: Non-A beta component of AD amyloid, NACP, PARK1, Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP)
Isotype: Rat IgG2a, κ