Biolegend, 867101, Purified anti-GRB2 Antibody, 25μg

Growth factor receptor bound protein 2 (GRB2) is an adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. GRB2 is ubiquitously expressed, and has been shown to be essential to various cellular functions. Due to its involvement in cell growth, cell proliferation and metabolism, GRB2 has been linked to multiple tumor malignancies, and thus is a potential therapeutic target for cancer therapy.  Recent neurodevelopmental studies also suggest that variations in intracellular levels of adapter proteins such as GRB2 may represent an underlying mechanism that links exogenous signals to cell fate determination.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human GRB2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2 µg per ml. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 2 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2810797 (BioLegend Cat. No. 867101) AB_2810798 (BioLegend Cat. No. 867102)
Structure: GRB2 is a 217 amino acid protein with predicted and observed molecular mass of ~25 kD.
Distribution: Tissue Distribution: Ubiquitously expressed Cellular Distribution: Plasma membrane, nucleus, cytosol, endosome, and Golgi apparatus
Function: GRB2 is an adapter protein that binds to activated SH2-containing proteins and plays an important role in determining cell fate.
Interaction: EGF receptors, PDGF receptors, and other proteins containing SH2 or SH3 domains.
Cell Type: Microglia, Neurons
Biology Area: Cancer Biomarkers, Cell Death, Neurodegeneration, Neuroscience, Signal Transduction
Molecular Family: Adaptor Proteins
Antigen References: Ijaz M, et al. 2018. Protein Pept Lett. 24(12):1084 Paff M, et al. 2014. Hum Vaccin Immunother. 10(11):3322 Belov AA and Mohammadi M. 2012. Sci Signal. 5(249):pe49
Gene ID: 28851478481504
UniProt: View information about GRB2 on UniProt.org
Clone: 5G3-E7-F11
Regulatory Status: RUO
Other Names: Growth Factor Receptor Bound Protein 2, ASH
Isotype: Mouse IgG1, κ