Biolegend, 867202, Purified anti-CKMT1 Antibody, 100μg

CKMT1 is the mitochondrial specific creatine kinase subunit that is ubiquitously expressed in mammals. CKMT1 is mainly localized to the mitochondrial intermembrane space and plays an important role in cellular energetics. Impairment in CKMT1 due to oxidative and radical-induced damage has been reported in numerous diseases including neurodegenerative disorders. Overexpression of CKMT1 is observed in several tumors in which high energy consumption is required for proliferation.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified recombinant human CKMT1 fragment expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Fihurka O, et al. 2023. Biomolecules. 13:. PubMed Fihurka O, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID: AB_2810799 (BioLegend Cat. No. 867201) AB_2810800 (BioLegend Cat. No. 867202)
Structure: CKMT1 is a 417 amino acid protein with predicted and observed molecular mass of ~47 kD.
Distribution: Tissue Distribution: Mainly expressed in brain, appendix, tonsil, skin, and colon Cellular Distribution: Mitochondrion and cytosol
Function: CKMT1 plays a central role in energy transduction in tissues with large, fluctuating energy demands such as the brain.
Interaction: CKMT1 exists as an octamer composed of four homodimers
Cell Type: Neurons
Biology Area: Cancer Biomarkers, Cell Death, Neurodegeneration, Neuroinflammation, Neuroscience
Molecular Family: Mitochondrial Markers
Antigen References: Gramage E, et al. 2013. Toxicology. 10.1016/j.tox.2013.02.013. Tanimura A, et al. 2014. PLoS One. 9(2):e89916. Onda T, et al. 2006. Br J Cancer. 94:698-709.
Gene ID: 11591271629593
UniProt: View information about CKMT1 on UniProt.org
Clone: 1A6-C7-G10
Regulatory Status: RUO
Other Names: CKMT1A, CKMT1B, Creatine Kinase, Mitochondrial 1A
Isotype: Mouse IgG1, κ