Biolegend, 867901, Purified anti-Ubiquilin-2 Antibody, 25μg

Ubiqulin-2 is a ubiquitin-like protein that facilitates the degradation of ubiquitinated proteins by mediating their binding to the proteasome. Mutations in ubiquilin-2 have been linked to neurodegenerative diseases such as amyotrophic lateral sclerosis and frontal temporal dementia. These mutations are linked to disruption and impairment in the delivery of ubiquitinated substrates to the proteasome. In addition, ubiquilin-2 can interact with components of the ERAD machinery and localizes to autophagosomes, indicating that it plays a role in these pathways.
25μg
Verified Reactivity: Human, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ubqln2 recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2810807 (BioLegend Cat. No. 867901) AB_2810808 (BioLegend Cat. No. 867902)
Structure: Human Ubiquilin-2 is a 624 amino acid protein with a predicted molecular mass of 65 kD and observed molecular mass of ~75 kD.
Distribution: Tissue distribution: Widely expressed, highest expression in cerebellar vermis Cellular distribution: Nucleus, cytoplasm, membrane, and autophagosome
Function: Ubiquilin-2 plays an important role in the regulation of different protein degradation pathways including the ubiquitin-proteasome system, autophagy, and the endoplasmic reticulum-associated protein degradation.
Interaction: Ubiquilin-2 interacts with UBQLN1, UBE3A, BTRC, 19S proteasome subunit, C9orf72, HNRNPA1, and HNRNPU.
Cell Type: Neurons
Biology Area: Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family: Autophagosome Markers
Antigen References: Deng HX. et al. 2011. Nature. 477(7363):211-5. Hjerpe R. et al. 2016. Cell. 166(4):935-949. Wu Q. et al. 2015. Acta Neuropathol. 129(3): 417–428.
Gene ID: 29978
UniProt: View information about Ubiquilin-2 on UniProt.org
Clone: A18118A
Regulatory Status: RUO
Other Names: Chap1, DSK2 homolog, Protein linking IAP with cytoskeleton 2, PLIC-2, hPLIC-2, Ubiquitin-like product Chap1/Dsk2
Isotype: Mouse IgG2b, κ