Biolegend, 868101, Purified anti-Cortactin (CTTN) Antibody, 25μg

Cortactin is a cytosolic protein that promotes polymerization, rearrangement of the actin cytoskeleton, and synaptogenesis. Stimuli such as alterations in the concentration of growth factors lead to phosphorylation of cortactin by tyrosine or serine/threonine kinases. This modification enables cortactin to bind to filamentous actin and Arp2/3, resulting in the branching of the actin cytoskeleton. In addition, cortactin may be involved in clathrin-independent endocytosis.  Amplification of the cortactin gene is observed in certain tumors, indicating that it may also be a component of tumorigenesis.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. For immunocytochemistry, a concentration range of 2 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2810811 (BioLegend Cat. No. 868101) AB_2810812 (BioLegend Cat. No. 868102)
Structure: Cortactin is a 634 amino acid protein with predicted molecular mass of ~ 71 kD and observed molecular mass of ~ 80 kD.
Distribution: Tissue Distribution: Ubiquitously expressed Cellular Distribution: Plasma membrane, cytoskeleton, cytosol, and Golgi apparatus
Function: Cortactin promotes polymerization and the rearrangement of the actin cytoskeleton upon external stimulations.
Interaction: SHANK2, SHANK3, FGD1, ABL2, KCNA2, PLXDC2, SRCIN1, SMASN1, ASAP1.
Cell Type: Astrocytes, Endothelial cells, Neurons, Oligodendrocytes
Biology Area: Cancer Biomarkers, Cell Motility/Cytoskeleton/Structure, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance, Synaptic Biology
Molecular Family: Cytoskeletal Proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References: Ramos-Garcia P, et al. 2019. Oral Dis. 25(4):949 Schnoor M, et al. 2018. Trends Cell Biol. 28(2):79 Yin M, et al. 2017. Oncotarget. 8(50):88232
Gene ID: 20171304360465
UniProt: View information about Cortactin on UniProt.org
Clone: 4F11/CTTN
Regulatory Status: RUO
Other Names: Amplaxin, Src Substrate Cortactin, Oncogene EMS1
Isotype: Mouse IgG1, κ