Biolegend, 869101, Purified anti-Musashi-1 Antibody, 25μg

Musashi-1 (MSI1) is an evolutionarily conserved RNA-binding protein with essential role in stem cell maintenance, nervous system development, and tumorigenesis. It was first identified in Drosophila as a protein required for the development of adult external sensory organs (sensilla) and neuronal potential in asymmetric cell division. In mammalian nervous systems, MSI1 is one of the key oncogenic factors for glioblastomas and medulloblastomas. The expression of MSI1 is associated with Notch signaling. The high expression of MSI1 is a marker of poor prognosis in medulloblastoma. Genomic analysis indicates that MSI1 functions as a master regulator of genes during development and tumor formation.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Musashi-1 recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. For immunohistochemistry, a concentration range of 0.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Chen L, et al. 2021. J Biol Chem. 297:101275. PubMed
RRID: AB_2814629 (BioLegend Cat. No. 869101) AB_2814630 (BioLegend Cat. No. 869102)
Structure: Human MSI1 is a 362 amino acid protein with a predicted and observed molecular mass of 39 kD.
Distribution: Tissue distribution: Central nervous system, fetal kidney, liver, lung, and pancreas Cellular distribution: Nucleus and cytoplasm
Function: MSI1 is an RNA binding protein that regulates the expression of target mRNAs at the translation level.
Cell Type: Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area: Cancer Biomarkers, Neurodegeneration, Neuroscience
Molecular Family: Enzymes and Regulators
Antigen References: Okabe M, et al. 2001. Nature. 411:94-8. de Sousa Abreu R. et al. 2009. J Biol Chem. 284(18):12125-35. Vo DT. et al. 2012. Am J Pathol. 181(5):1762-72. Sengupta U. et al. 2018. Acta Neuropathol Commun. 6(1):113.
Gene ID: 4440
UniProt: View information about Musashi-1 on UniProt.org
Clone: 3F8-E6-B9
Regulatory Status: RUO
Other Names: RNA-binding protein Musashi homolog 1, Musashi-1, MSI1, Musashi RNA Binding Protein 1
Isotype: Mouse IgG2b, κ