Biolegend, 869602, Purified anti-Gemin 4 Antibody, 100μg

Gemin 4 is one of the core components of the survival of motor neurons (SMNs) macromolecule protein complex, which plays an important role in the assembly and re-structuring of small nucleoproteins and transcriptosomes. Recent studies have shown that one of the three putative nuclear localization signal motifs in Gemin 4 is necessary and sufficient to direct the nuclear import of the other two SMN complex components, Gemin 2 and Gemin 3. This suggests that Gemin 4 plays an essential role in mammalian snRNP biogenesis via facilitating the import of the SMN complex or subunits into the nucleus.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Histidine-tagged recombinant protein corresponding to human Gemin 4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Battle DJ, et al. 2007. J. Biol Chem. 282(38):27953 (WB)
RRID: AB_2814637 (BioLegend Cat. No. 869601) AB_2814638 (BioLegend Cat. No. 869602)
Structure: Gemin 4 is a 1058 amino acid protein with predicted and observed molecular mass of ~120 kD.
Distribution: Tissue Distribution: Ubiquitously expressed Cellular Distribution: Nucleus and cytosol
Function: Gemin 4 is a component of the SMN macromolecule protein complex.
Interaction: Gemin 4 is a part of the core SMN complex that contains SMN 1-8.
Biology Area: Neuroscience, Synaptic Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Meier ID, et al. 2018. Biol Open. 7(2) Battle DJ, et al. 2007. J Biol Chem. 282(38):27953 Wang JL, et al. 2004. Biochim Biophys Acta. 1673(1-2):75.
Gene ID: 50628
UniProt: View information about Gemin 4 on UniProt.org
Clone: 17D10
Regulatory Status: RUO
Other Names: P97, Gem Nuclear Organelle Associated Protein 4, Component of Gems 4, Gem-Associated Protein 4, HCC-Associated Protein 1, Gemin-4, Gem (Nuclear Organelle) Associated Protein 4
Isotype: Mouse IgG1, κ