Biolegend, 869802, Purified anti-Lamin B1 Antibody, 100μg

Lamin B1 is a type V intermediate filament and an integral component of the nuclear lamina, a meshwork of proteins located inside the nuclear membrane. There are 3 major types of nuclear lamin proteins: lamin A/C, lamin B1 and lamin B2. Lamin A and C are the alternative splicing products of LMNA gene, whereas lamin B1 and lamin B2 are encoded by genes LMNB1 and LMNB2, respectively. Lamins are crucial for maintaining the nuclear structure. They are also important for regulating nuclear functions and activities, including DNA replication and transcription, nuclear and chromatin organization, cell cycle regulation, cell development and differentiation, nuclear migration, and apoptosis. In the brain, lamin B1 levels modulate brain development. Loss of lamin B1 in mouse adversely impairs embryonic brain development and corticogenesis. In addition, duplications of LMNB1 gene causes autosome-dominant leukodystrophy, an adult-onset demyelinating disorder characterized by symmetrical widespread myelin loss in the central nervous system with a phenotype similar to chronic progressive multiple sclerosis.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Lamin B1 protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Strine MS, et al. 2023. PLoS Biol. 21:e3002097. PubMed
RRID: AB_2820180 (BioLegend Cat. No. 869801) AB_2820181 (BioLegend Cat. No. 869802)
Structure: Human lamin B1 is a 586 amino acid protein with a predicted molecular mass of 66 kD.
Distribution: Tissue distribution: Ubiquitously expressed Cellular distribution: Nucleus
Function: Lamin B1 is a component of the nuclear lamina, providing a framework for nuclear envelope.
Interaction: Lamin B1 interacts with lamin-associated peptides IA, IB, 2, and SPAG4 and SEPT12.
Cell Type: Astrocytes, Microglia, Neurons, Oligodendrocytes
Biology Area: Cell Biology, Cell Structure, Chromatin Remodeling/Epigenetics, Neurodegeneration, Neuroinflammation, Neuroscience
Molecular Family: Nuclear Markers
Antigen References: Padiath QS. et al. 2006. Nat Genet. 38(10):1114-23. Camps J. et al. 2015. Nucleus. 6(1):8-14. Mahajani S. et al. 2017. Sci Rep. 7(1):4897.
Gene ID: 4001
UniProt: View information about Lamin B1 on UniProt.org
Clone: 5G8-D3-H7
Regulatory Status: RUO
Other Names: LMN2, LMNB, ADLD, LMN
Isotype: Mouse IgG2b, κ