Biolegend, 869902, Purified anti-Complement C5b Antibody, 100μg

The complement system is a part of eukaryotic innate defense system that enhances the clearance of exogenous microbes and damaged cells by antibodies and phagocytic cells. In vivo , the complement system is activated by three pathways: the classical complement pathway, the alternative complement pathway, and the lectin pathway. Complement C5 is a 1676 amino acid precursor protein synthesized in the liver. All three complement activation pathways culminate in the cleavage of C5, which produces two disulfide linked glycoproteins C5α and C5β. C5α is further cleaved by C5 convertase into C5a and C5α', the latter binds to C5β to form C5b, which in turn sequentially recruits C6-C9 to form the membrane attack complex (MAC). MAC destroys pathogens by forming a lytic pore in their membrane. Mutations affecting the Complement C5 gene lead to complement component 5 deficiency where patients demonstrate an increased propensity to develop severe recurrent infections.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH conjugated synthetic peptide corresponding to amino acids 428–454 of human complement C5 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 2.0 - 10 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody predominantly detects C5b in human cell line and human plasma. At high concentration (between 2 and 10 µg/mL), the full-length C5 is also detected at ~190 kD in human cell lines, but not in human plasma.
RRID: AB_2820182 (BioLegend Cat. No. 869901) AB_2820183 (BioLegend Cat. No. 869902)
Structure: C5b is a 655 amino acid protein with predicted and observed molecular mass of ~ 75 kD.
Distribution: Tissue Distribution: Predominantly in liver and plasma. Also expressed in the brain. Cellular Distribution: Plasma membrane and extracellular
Function: Complement C5b generation and assembly of MAC facilitates the lysis and destruction of pathogens.
Interaction: Interacts with C5a chain
Ligand/Receptor: Staphylococus aureus protein SSL5, binds C6 (transiently)
Cell Type: Astrocytes, Mature Neurons, Microglia
Biology Area: Cell Biology, Complement, Neurodegeneration, Neuroinflammation, Neuroscience
Molecular Family: Innate Immune Signaling
Antigen References: Vlaicu SI, et al. 2019. Front Immunol. 10:1054 Fishelson Z, et al. 2019. Front Immunol. 10:752 Bergseth G, et al. 2013. Mol Immunol. 56(3):232
Gene ID: 727
UniProt: View information about Complement C5b on UniProt.org
Clone: A18119A
Regulatory Status: RUO
Other Names: complement C5 beta chain, C5a
Isotype: Mouse IgM, κ