Biolegend, 870101, Purified anti-Optineurin Antibody, 25μg

Optineurin (OPTN), also known as FIP-2 or NEMO-related protein (NRP), is a cytoplasmic protein associated with diverse cellular functions including membrane trafficking, vesicle transport, and cell cycle control. OPTN has also been identified as an autophagy receptor important in elimination of damaged mitochondria through mitophagy, a selective macroautophagy process. Mutations in the Optn gene have been linked to a variety of disorders including amyotrophic lateral sclerosis, glaucoma, and Paget's disease of bone. In vitro cellular studies suggest that optineurin recognizes various protein aggregates via its C-terminal coiled-coiled domain in a ubiquitin-independent manner and facilitates autophagic clearance of protein aggregates. Immunohistochemical analyses demonstrate the localization of OPTN to pathological tau tangles in Alzheimer's disease tissues, and Lewy bodies and neurites in Parkinson's disease tissues, supporting its role in neurodegenerative diseases.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human optineurin protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 2.0 - 10 µg/mL. For immunocytochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Duan K, et al. 2021. Neuron. 109:3793. PubMed
RRID: AB_2820186 (BioLegend Cat. No. 870101) AB_2820187 (BioLegend Cat. No. 870102)
Structure: Human optineurin is a 577 amino acid protein with a predicted molecular mass of 66 kD and an observed molecular mass of 74 kD.
Distribution: Tissue distribution: Widely expressed in tissues Cellular distribution: Nucleus, endosome, cytosol, Golgi apparatus, mitochondrion, lysosome
Function: Optineurin plays an important role in the maintenance of the Golgi complex, membrane trafficking and exocytosis.
Interaction: Interacts with myosin VI and Rab8
Cell Type: Astrocytes, Neurons
Biology Area: Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Molecular Family: Adaptor Proteins
Antigen References: Osawa T, et al. 2011. Neuropathology. 31:569-74. Ying H, Yue BY. 2012. Int. Rev. Cell. Mol. Biol. 294: 223-58. Korac J, et al. 2013. J. Cell. Sci. 126: 580-92.
Gene ID: 10133
UniProt: View information about Optineurin on UniProt.org
Clone: A18152B
Regulatory Status: RUO
Other Names: E3-14.7K-interacting protein, FIP-2, Huntingtin yeast partner L, Huntingtin-interacting protein 7, HIP-7, NEMO-related protein, Optic neuropathy-inducing protein, Transcription factor IIIA-interacting protein, TFIIIA-IntP
Isotype: Mouse IgG2a, κ