Biolegend, 870201, Purified anti-UBE2O Antibody, 25μg

Ubiquitin-conjugating enzyme E2 O (UBE2O) is an E2/E3 hybrid enzyme that displays both E2 and E3 ligases activities and mediates ubiquitination of many target proteins involved in a wide range of processes, including the 5’-AMP-activated protein kinase catalytic subunit a2 (AMPKa2), tumor suppressor ubiquitin carboxyl-terminal hydrolase BAP1, mixed-lineage leukemia (MLL) protein, SMAD family member 6 (SMAD6), transcription factor c-Maf, and free ribosomal proteins. Abnormal expression and mutations in UBE2O are observed in tumorigenesis and are associated with poor prognosis of gastric, lung, breast, and prostate cancers.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human UBE2O protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.2 - 5.0 µg/mL. For immunocytochemistry, a concentration range of 0.5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2820188 (BioLegend Cat. No. 870201) AB_2820189 (BioLegend Cat. No. 870202)
Structure: Human UBE2O is a 1,292 amino acid protein with a predicted molecular mass of 141 kD and observed molecular mass of ~200 kD
Distribution: Tissue distribution: Brain, skeletal muscle, heart Cellular distribution: Nucleus, cytosol
Function: UBE2O is an E2/E3 hybrid ubiquitin-protein ligase that is crucial for protein ubiquitination and degradation.
Cell Type: Astrocytes, Microglia, Neurons, Oligodendrocytes
Biology Area: Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Ubiquitin/Protein Degradation
Molecular Family: Ligases
Antigen References: Ullah K, et al. 2019. FEBS J. 286(11):2018-2034. Chen S, et al. 2018. J Biol Chem. 293(29):11296-11309. Mashtalir N, et al. 2014. Mol Cell. 54(3):392-406.
Gene ID: 63893
UniProt: View information about UBE2O on UniProt.org
Clone: A18140A
Regulatory Status: RUO
Other Names: (E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, Ubiquitin carrier protein O, Ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kD, Ubiquitin-protein ligase O
Isotype: Mouse IgG2b, κ