Biolegend, 870401, Purified anti-Parkin Antibody, 25μg

Parkin (also known as PARK2, AR-JP, PRKN) is a protein encoded by the PARK2 gene in humans. It is a component of the E3 ubiquitin ligase complex that mediates the targeting of proteins for degradation. Mutations in the PARK2 gene cause a familial form of Parkinson’s disease called autosomal recessive juvenile parkinsonism (AR-JP).
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant full-length human Parkin protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Tokarew, J., et al. 2021. Acta Neuropathologica 2021. 141(5): 725-754. PubMed
RRID: AB_2820192 (BioLegend Cat. No. 870401) AB_2820193 (BioLegend Cat. No. 870402)
Structure: Human Parkin is a 465 amino acid protein with a predicted molecular mass of ~52 kD.
Distribution: Tissue distribution: Brain, heart, testis, and skeletal muscle Cellular distribution: Cytosol, mitochondria, nucleus, and endoplasmic reticulum
Function: Parkin is involved in protein ubiquitination, protein degradation, mitochondria function, and neurodegeneration.
Interaction: Ubiquitin, PINK1
Ligand/Receptor: BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1
Cell Type: Neurons
Biology Area: Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Ubiquitin/Protein Degradation
Molecular Family: Ligases
Antigen References: Tay SP, et al. 2010. J Biol Chem. 285: 29231-8. Pawlyk AC, et al. 2003. J Biol Chem. 278(48):48120-8.
Gene ID: 5071
UniProt: View information about Parkin on UniProt.org
Clone: A15165E
Regulatory Status: RUO
Other Names: PARK2, AR-JP, LPRS2, PDJ, PRKN, E3 ubiquitin-protein ligase parkin, Parkin 2, E3 ubiquitin ligase, parkinson disease protein 2, parkinson juvenile disease protein 2, Parkinson disease (autosomal recessive, juvenile) 2
Isotype: Mouse IgG2b, κ