Biolegend, 870601, Purified anti-FAF1 Antibody, 25μg

FAS-associated factor 1 (FAF1) is an evolutionarily conserved protein with multiple protein interaction domains, including a FAS-interacting domain (FID), a death effector domain interacting domain (DEDID), and multi-ubiquitin-related domains. Initially identified as a member of FAS death-inducing signaling complex, FAF1 plays diverse roles in apoptotic and survival pathways. It may also serve as a scaffolding protein that regulates protein degradation in the ubiquitin-proteasome pathway. FAF1 is abundantly expressed in the brain where it modulates cell death. FAF1 also serves as a substrate for parkin, a key protein implicated in the pathogenesis of Parkinson’s disease (PD). Wild type parkin ubiquitinates FAF1 and promotes its degradation, while PD-linked mutant parkin causes abnormal accumulation of FAF1.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant full length protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2820196 (BioLegend Cat. No. 870601) AB_2820197 (BioLegend Cat. No. 870602)
Structure: Human FAF1 is a 650 amino acid protein with a predicted molecular mass of 74 kD.
Distribution: Tissue distribution: Ubiquitously expressed Cellular distribution: Plasma membrane, nucleus, cytosol, endoplasmic reticulum
Function: FAF1 is an ubiquitin-binding protein, required for the progression of DNA replication forks.
Interaction: Interacts with CDT1, ATPase VCP/p97, FAS, and NLRP12.
Cell Type: Neurons
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Cycle/DNA Replication, Cell Death, Neuroscience, Ubiquitin/Protein Degradation
Antigen References: Menges CW, et al. 2009. Cell Cycle. 8(16):2528-34. Sul JW, et al. 2013. Hum Mol Genet. 22(8):1558-73. Cheng K, et al. 2011. J Neurosci. 31(33):11905-13.
Gene ID: 11124
UniProt: View information about FAF1 on UniProt.org
Clone: CPTC-FAF1-1
Regulatory Status: RUO
Other Names: FAS-associated factor 1, hFAF1, UBX domain-containing protein 12, UBX domain-containing protein 3A, UBXD12, UBXN3A, Fas (TNFRSF6) Associated Factor 1, TNFRSF6-Associated Factor 1
Isotype: Mouse IgG1, κ