Biolegend, 870902, Purified anti-VCP Antibody, 100μg

Valocin-containing protein (VCP) is a member of a family of proteins that include putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP associates with clathrin and heat-shock protein Hsc70 to form a complex that functions as a chaperon to unfold and transport proteins to different cellular compartments. Diseases associated with VCP include inclusion body myopathy with early-onset Paget's disease, and frontotemporal dementia 1 and amyotrophic lateral sclerosis 14, with or without frontotemporal dementia.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human VCP Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2861105 (BioLegend Cat. No. 870901) AB_2861106 (BioLegend Cat. No. 870902)
Structure: VCP is an 806 amino acid protein with predicted molecular mass of ~89 kD and observed molecular mass at ~ 97 kD.
Distribution: Tissue Distribution: Ubiquitously expressed Cellular Distribution: Cytosol, endoplasmic reticulum, nucleus, extracellular, and lysosome
Function: Protein Trafficking, protein clearance
Interaction: Forms homohexamers and a ternary complex with STX5A and NSFLC. Interacts with NPLOC4, UFD1, and clathrin.
Cell Type: Astrocytes, Dopaminergic Neurons, Endothelial cells, Epithelial cells, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area: Cell Motility/Cytoskeleton/Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Signal Transduction, Ubiquitin/Protein Degradation
Molecular Family: Endoplasmic Reticulum Markers, Neurotransmitter Receptor Trafficking/Endocytosis, Synaptic Vesicle Trafficking/Endocytosis
Antigen References: Lan B, et al. 2017. Curr Mol Med. 17(9):608. van den Boom J & Meyer H. 2018. Mol Cell. 69(2):182. Van Mossevelde S, et al. 2018. Nat Rev Neurol. 14(6):363.
Gene ID: 7415269523116643
UniProt: View information about VCP on UniProt.org
Clone: A18143C
Regulatory Status: RUO
Other Names: Valosin-Containing Protein, CDC48
Isotype: Mouse IgG2b, κ