Biolegend, 871002, Purified anti-BRAP Antibody, 100μg

BRCA1 associated protein (BRAP) is a cytosolic protein that negatively regulates MAP kinase activation by inhibiting the formation of the Raf/MEK complex. BRAP also inhibits nuclear translocation and promotes the retention of several proteins, including BRCA1, in the cytoplasm by binding to their nuclear localization signal sequence.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified recombinant human BRAP protein fragments expressed in E. coli
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2832876 (BioLegend Cat. No. 871001) AB_2832877 (BioLegend Cat. No. 871002)
Structure: BRAP is an 592 amino acid protein with predicted and observed molecular mass of ~67 kD.
Distribution: Tissue Distribution: Ubiquitously expressed Cellular Distribution: Nucleus, Cytosol
Function: Protein trafficking, Epigenetics
Interaction: BRCA1, Galectin-2
Cell Type: Astrocytes, Dopaminergic Neurons, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area: Cell Biology, Innate Immunity, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance, Ubiquitin/Protein Degradation
Molecular Family: Innate Immune Signaling
Antigen References: Modell SM, et al. 2014. J Relig Health. 53(3):715. Castro E & Eeles R. 2012. Asian J Androl. 14(3):409. Pristauz G, et al. 2010. Wien Med Wochenschr. 160(7-8):158.
Gene ID: 831572399
UniProt: View information about BRAP on UniProt.org
Clone: 1E7-C9-D10-B10
Regulatory Status: RUO
Other Names: BRCA1 Associated Protein
Isotype: Mouse IgG1, κ