Biolegend, 899901, Autophagy Antibody Sampler Kit, 1kit

Autophagy is a regulated process for protein degradation and turnover of damaged organelles to maintain cellular homeostasis. Under conditions such as stress or starvation, autophagy contributes to the breakdown of proteins and cellular compartments to generate amino acids required for the synthesis of proteins essential for cell survival. Dysregulation of autophagy or mutations in proteins involved in this pathway have been shown to contribute to neurodegenerative disorders such as Parkinson’s disease. The Autophagy Antibody Sampler Kit provides flexibility for sampling and detection of key targets in the autophagy pathway.
1kit
Formulation: Please refer to individual product datasheets of the purified formats for details.
Preparation: All antibodies in this kit were purified by affinity chromatography.
Storage & Handling: Upon receipt, store undiluted at 2-8°C.
Application: WB, IHC-P - Quality tested ICC - Verified
Recommended Usage: Each lot of antibodies in this kit is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows: Anti-Ubiquitin: 1.0 - 10 µg/ml Anti-Rab7A: 0.001 - 0.005 µg/ml Anti-Beclin-1: 0.5 - 5.0 µg/ml Anti-ATG5: 0.1 - 1.0 µg/ml For immunohistochemistry, the suggested uses of these reagents are as follows: Anti-ATG5: 0.2 - 1.0 µg/ml Anti-LC3: 0.5 - 1.0 µg/ml It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For verified or reported applications for these antibodies, please see individual product datasheets.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Antigen References: Uchiyama Y, et al. 2008. Histochem. Cell Biol. 129:407. Wu H, et al. 2014. Int. J. Biol. Sci. 10:1072. Subramani S, Malhotra V. 2013. EMBO Rep. 14(2):143. Cao Y, et al. 2007. Cell. Research 17:839. Lee J, et al. 2012. Biochem. J. 441(2):523. Martini-Stoica H, et al. 2016. Trends Neurosci. 39(4)221. Guerra F, Bucci C. 2016. Cells. 5(3). Hatanaka H, et al. 2009. J. Biol. Chem. 23:15448.
Gene ID: 947486788163178797316
Regulatory Status: RUO
Other Names: Please refer to individual product datasheets.