Biolegend, 901902, Purified anti-AU1 Epitope Tag Antibody (Previously Covance catalog# MMS-130P), 500microl

The AU1 tag, which consists of the peptide sequence DTYRYI, is often used as a protein modification in order to simplify the labeling and detection of proteins. This unique amino acid sequence allows for specific antibody detection in western blotting and other immunostaining techniques. Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.
500microl
Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Monoclonal antibody AU1 was raised against an SDS-disrupted BPV-1 preparation and mapped to the six amino acid epitope DTYRYI.
Formulation: Phosphate-buffered solution + 0.03% Thimerosal.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application: WB, ICC, IP, Purification
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. The optimal working dilution should be determined for each specific assay condition. • WB: 1:1,000 • ICC: 1:1,000 • IP: 1:150
Application Notes: This antibody is effective in immunofluorescence (IF), immunoblotting (WB), and immunoprecipitation (IP) of tagged proteins. The clone was specifically designed for use in epitope tagging. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence. This antibody was purified using protein-G chromatography.
Application References(PubMed link indicates BioLegend citation): Mothe-Satney I, et al. 2000. J Biol Chem. 275:33836. Goldstein DJ, et al. 1992. EMBO J. 11:4851. Lim PS, et al. 1990. J Infect Dis. 162:1263. Sauter D, et al. 2011. PLoS Pathog. 8:e1003093. (WB, IF) Kluge SF, et al. 2013. Retrovirology 10:32. (WB, IF) PubMed Heigele A, et al. 2012. J. Virol. 86:36. WB) PubMed
Product Citations: Heigele A, et al. 2012. J Virol. 86:36-48. PubMed Waise TMZ, et al. 2019. Nat Commun. 10:714. PubMed Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed Kluge S, et al. 2013. Retrovirology. 10:32. PubMed Kim YJ, et al. 2021. PLoS Pathog. 17:e1009179. PubMed Joas S, et al. 2020. Cell Rep. 30:2261. PubMed Chen X, et al. 2021. Sci Rep. 11:14525. PubMed
RRID: AB_2734667 (BioLegend Cat. No. 901905) AB_2565013 (BioLegend Cat. No. 901901) AB_2565014 (BioLegend Cat. No. 901902)
Biology Area: Cell Biology
Gene ID: NA
UniProt: View information about AU1 on UniProt.org
Clone: AU1
Regulatory Status: RUO
Other Names: AU1 epitope tag, DTYRYI epitope tag, DTYRYI tag
Previously: Covance Catalog# MMS-130P
Isotype: Mouse IgG2a