Biolegend, 904402, Purified anti-c-Myc Antibody (Previously Covance catalog# MMS-164P), 25microl

c-Myc truncations are often used as an epitope tag in order to simplify the labeling and detection of proteins. This antibody recognizes the c-Myc epitope tag and fused recombinant proteins allowing for specific detection in western blotting, immunoprecipitation, and immunostaining techniques.
25microl
Verified Reactivity: All Species
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution + 0.03% Thimerosal.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application: WB - Quality tested ICC, IHC-P, IP - Verified Purification - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The 9E11 monoclonal antibody recognizes the sequence 408-420 AEEQKLISEEDL of human c-myc. This clone recognizes a non-specific band in BL21 E. coli lysates BioLegend offers two clones against c-Myc, 9E10 and 9E11. When both clones were tested in parallel for WB, ICC, and IP, each clone performed similarly, with 9E11 showing slightly better performance for each application.
Additional Product Notes: This antibody recognizes endogenous human c-Myc protein in IHC-P. Reactivity with endogenous c-Myc in other applications is not verified.
Application References(PubMed link indicates BioLegend citation): Halme A, et al. 2004. Cell. 116:405. Kolodziej P, Young R. 1991. Meth. Enzymol. 194:508. Locker AP, et al. 1989. Brit. J. Cancer. 60:669.
Product Citations: Sims S, et al. 2021. Adv Virol. 2021:5569844. PubMed Yi B, et al. 2022. Oncogene. 41:268. PubMed Wu Y, et al. 2016. Nat Struct Mol Biol. 10.1038/nsmb.3284. PubMed
RRID: AB_2565039 (BioLegend Cat. No. 904401)
Biology Area: Cell Biology, Protein Purification, Protein Synthesis
Gene ID: 4609
UniProt: View information about c-Myc on UniProt.org
Clone: 9E11
Regulatory Status: RUO
Other Names: myc tag, c-Myc tag, cMyc tag, Myc epitope tag
Previously: Covance Catalog# MMS-164P
Isotype: Mouse IgG2a, κ