Biolegend, 904804, Purified anti-Cytokeratin 8 Antibody, 100μg

Cytokeratin 8 copolymerizes with cytokeratin 18 to form a component of the cytoskeleton in simple epithelia and many epithelial cell derived cancers. It is a member of the Type II keratin family clustered on the long arm of chromosome 12.
100μg
Verified Reactivity: Human, Mouse
Reported Reactivity: Dog, Non-Human Primate
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was raised against the 12 carboxy-terminal amino acids of HK-8 preceded by a cysteine, CKLVSESSDVLPK.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 10 μg/ml is suggested. For Western blotting, a concentration range of 0.5 - 5.0 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy4.
Application References(PubMed link indicates BioLegend citation): Memarzadeh S, et al. 2010. Proc. Natl. Acad. Sci. USA 107:17298. PubMed Hembrough TA. 1996. J. Biol. Chem. 271:25684. PubMed Hübner A, et al. 2012. Proc. Natl. Acad. Sci. USA 109:12046. PubMed Easter SL, et al. 2014. PLoS One 9(11):e113247. (IF) PubMed
Product Citations: Okada N, et al. 2023. Commun Biol. 6:596. PubMed Fujimori S, et al. 2022. Elife. 11:. PubMed Amonkar GM, et al. 2022. STAR Protoc. 3:101390. PubMed Yip HYK, et al. 2021. STAR Protocols. 2(3):100765. PubMed Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed Yip HYK, et al. 2020. Molecular Cell. 80(2):279-295.e8. PubMed Mao X, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID: AB_2616821 (BioLegend Cat. No. 904804)
Structure: Cytokeratin 8 is a 483 amino acid protein with a molecular mass of 54 kD.
Distribution: Tissue distribution: colon, small intestine, pancreas, liver, stomach, and salivary glands. Cellular distribution: extracellular and nucleus.
Function: Cytokeratin 8 plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Together with KRT19, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Gene ID: 3856
UniProt: View information about Cytokeratin 8 on UniProt.org
Clone: 1E8
Regulatory Status: RUO
Other Names: Keratin, type II cytoskeletal 8, CK-8, keratin-8, cytokeratin-8, K8
Isotype: Mouse IgG2a, κ