Biolegend, 904901, Purified anti-Cytoplasmic Dynein Intermediate Chain Antibody (Previously Covance catalog# MMS-400P), 100microl

Dyneins are motor proteins that transport various cellular cargo along cytoskeletal microtubules in eukaryotic cells. Dynein has the same basic structures in all cell types and is composed of two or three distinct heavy chains (450 kD), three intermediate chains (70-125 kD), and at least four light chains (15-25 kD).  The cytoplasmic dynein intermediate chain is thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function.
100microl
Verified Reactivity: Human, Mouse
Reported Reactivity: Other species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was raised against purified bovine brain cytoplasmic dynein.
Formulation: Phosphate-buffered solution + 0.03% Thimerosal.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml (1:1000). For immunocytochemistry, a concentration range of 4.0 - 10.0 μg/ml (1:100-1:250) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is effective in immunohistochemistry (IHC), immunoblotting and immunoprecipitation (IP). *Predicted MW = 74 kD. This antibody is known not to react in squid and nematode. Clone 74.1 reacts with all isoforms from both rat IC74 genes. The antigenic site is believed to be located in the conserved amino-terminal 60 amino acids. Clone 74.1 has been shown to inhibit the activity of cytoplasmic dynein.
Application References(PubMed link indicates BioLegend citation): Miller MG, et al. 1999. Biol Reprod. 60:1047. Dillman JF, et al. 1996. Proc Natl Acad Sci USA. 93:141. Pfister KK, et al. 1996.J Biol Chem. 271:1687. Dillman JF, et al. 1994. J Cell Biol. 127:1671. Combs J, et al. 2006. PNAS. 103:14883. Chen JL, et al. 2005. J Cell Biol. 169:383. (IP) PubMed Burakov A, et al. 2008. Traffic. 9:472. Payne C, et al. 2003. J Cell Sci. 116:4727. (ICC) PubMed Semenova I, et al. 2014. Mol Biol Cell. 25:3119. (WB) PubMed
Product Citations: Dwivedi D, et al. 2019. J Cell Biol. 218:871. PubMed Payne C, et al. 2003. J Cell Sci. 116:4727-4738. PubMed Chen J, et al. 2005. J Cell Biol. 169:383-389. PubMed Nath S, et al. 2016. J Immunol. 197: 2090 - 2101. PubMed Sabblah TT, et al. 2018. Sci Rep. 1.540972222. PubMed Zhang J, et al. 2016. Sci Rep. 6:37050. PubMed Semenova I, et al. 2014. Mol Biol Cell. 25:3119-3132. PubMed
RRID: AB_2565044 (BioLegend Cat. No. 904901)
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Gene ID: 83658
UniProt: View information about Cytoplasmic Dynein Intermediate Chain on UniProt.org
Clone: 74.1
Regulatory Status: RUO
Previously: Covance Catalog# MMS-400P
Isotype: Mouse IgG2b, κ