Biolegend, 906109, Direct-Blot™ HRP anti-6-His Epitope Tag Antibody, 25microl

His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).
25microl
Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
Purity: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 5.0 µg per ml (1:1000-1:10000 dilution). It is recommended that the reagent be titrated for optimal performance for each application. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
Application Notes: The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.
Application References(PubMed link indicates BioLegend citation): Deinert K, et al. 2001. J. Biol. Chem. 276:6000. (WB) Kojima H, et al. 2001. J. Biol. Chem.(22)276:19238. (ICC) PubMed Lapinski PE, et al. 2000. J. Biol. Chem. 275:6831. (WB) Bartsch D, et al. 1998. Cell. 95:211. (ICC) Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed Montegna EA, et al. 2012 PLoS One.7:31156. (WB) PubMed Huang L, et al. 2013. Nat Biotechnol. 31:450. (WB) PubMed
Product Citations: Chandrasekar SS, et al. 2021. Vaccines (Basel). 9:. PubMed
RRID: AB_2734681 (BioLegend Cat. No. 906109) AB_2734682 (BioLegend Cat. No. 906110)
Biology Area: Cell Biology
Gene ID: NA
UniProt: View information about 6-His on UniProt.org
Clone: 6-His
Regulatory Status: RUO
Other Names: polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
Isotype: Mouse IgG1, κ