Biolegend, 908806, Biotin anti-c-Myc Antibody (Previously Covance catalog# BIOT-150L), 25microl

The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.
25microl
Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Amino acids 408-439, C-terminal region of human c-myc
Formulation: Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.4 µg per ml (1:2500 dilution). For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/ml (1:1000-1:2000) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: *Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
Application References(PubMed link indicates BioLegend citation): Rao H, et al. 2001. Nature. 410:955. Peters CS, et al. 2001. J Biol Chem. 276:13718. Munro S, Pelham HR. 1987. Cell. 48:899. Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
Product Citations: Rad S M AH, et al. 2020. PLoS One. 15:e0232915. PubMed Tan GMY, et al. 2022. Cancers (Basel). 14:. PubMed Marshall RS, et al. 2020. J Cell Sci. 133:jcs249862. PubMed Parodi E, et al. 2015. PLoS One. 10: 0143571. PubMed Marshall RS, et al. 2020. J Cell Sci. :133. PubMed
RRID: AB_2565090 (BioLegend Cat. No. 908805)
Biology Area: Cell Biology, Protein Purification, Protein Synthesis
Antigen References: Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217. Battey J, et al. 1983. Cell 34:779. Beimling P, et al. 1985. Biochemistry 24:6349.
Gene ID: 4609
UniProt: View information about c-Myc on UniProt.org
Clone: 9E10
Regulatory Status: RUO
Other Names: myc tag, c-Myc tag, cMyc tag, Myc epitope tag
Previously: Covance Catalog# BIOT-150L
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.