Biolegend, 914212, Alexa Fluor® 647 anti-Pan-Cytokeratin Antibody, 100μg

AE-1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with molecular weights of 56.5, 50, 48 and 40 kD. AE-3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including cytokeratins with molecular weights of of 64, 59, 58, 56, and 52 kD.
100μg
Verified Reactivity: Human, Rat
Reported Reactivity: Dog, Non-Human Primate
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was developed using human epidermal keratins.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1 - 10 μg/mL is recommended. For flow cytometric staining using our Cyto-Fast™ Fix/Perm Buffer Set, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Additional Product Notes: For use of this antibody (clone AE-1/AE-3) in immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with either 0.5% Triton-X or 100% ice-cold methanol, or fixation/permeabilization with 100% ice-cold methanol alone. For use of this antibody (clone AE-1/AE-3) in intracellular flow cytometry (ICFC), we recommend fixation/permeabilization using Cyto-FastTM Fix/Perm Buffer Set (Cat. No. 426803). For use of this antibody (clone AE-1/AE-3) in immunohistochemistry (IHC), we recommend antigen retrieval with either Sodium Citrate H.I.E.R. (Cat. No. 928502) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422703).
RRID: AB_3675197 (BioLegend Cat. No. 914211) AB_3675197 (BioLegend Cat. No. 914212)
Distribution: Cytoplasm, cell membrane
Function: Cytoskeleton, cell motility and structure
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Antigen References: Metcalf KJ, et al. 2022. Matrix Biol Plus. 14:100105. Jiang S, et al. 2022. Immunity. 55:1118. Tiago M, et al. 2020. Br J Cancer. 122:789. Kou R, et al. 2018. Oncol Rep. 7:2013. Xie S, et al. 2020. Ann Transl Med. 1.384027778. Chang TH, et al. 2020. Cell Death Dis. 11:338. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. Cai S, et al. 2022. iScience. 25:104980.
Gene ID: NA
UniProt: View information about Pan-Cytokeratin on UniProt.org
Clone: AE-1/AE-3
Regulatory Status: RUO
Other Names: Pan-Cytokeratin (AE-1/AE-3), AE1/AE3
Isotype: Mouse IgG1, κ