Biolegend, 919101, Purified anti-Vimentin Antibody (Previously Covance catalog# PCK-594P), 100microl

Vimentin is a protein subunit of the 10nm or intermediate filaments protein found in many kinds of mesenchymal and epithelia cells, several types of cells in tissue culture and in developing neuronal and astrocytic precursor cells in the central nervous system. Vimentin frequently forms copolymers with other intermediate filament proteins, such as GFAP (in many kinds of astrocytes), with desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton.
100microl
Verified Reactivity: Human, Rat, Mouse
Antibody Type: Polyclonal
Host Species: Chicken
Immunogen: This antibody was raised in chickens against purified recombinant vimentin.
Formulation: Phosphate-buffered solution, containing 10 mM sodium azide.
Preparation: The antibody is an IgY preparation.
Concentration: ~20 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application: IHC-P - Quality tested WB, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry, a dilution range of 1:200 - 1:500 is suggested. For Western blotting, the suggested use of this reagent is 1:2000 - 1:8000 dilution. For immunocytochemistry, a dilution range of 1:1000 - 1:5000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is effective in immunofluorescence (IF) and immunohistochemistry (IHC). This antibody does not bind to protein A or protein G. Predicted MW is ~54 kD
Application References(PubMed link indicates BioLegend citation): Herrmann H, et. al. 1989. Development 105:279. (IF, ICC, IHC, WB) KivelS T, et. al. 1992. J. Histochem. Cytochem. 40:1517. (IHC) Stathopoulos, et. al. 1989. J. Histochem. Cytochem. 37:1363. (IHC)
Product Citations: Lee DH, et al. 2019. PLoS One. 14:e0211153. PubMed Almada P, et al. 2019. Nat Commun. 10:1223. PubMed Heinrich A, et al. 2021. Cell Rep. 37:109885. PubMed Mi J, et al. 2018. J Biol Chem. 293:20123. PubMed Kittisopikul M, et al. 2020. Bioinformatics. 36:5093. PubMed Robert A, et al. 2015. Proc Natl Acad Sci U S A. 112: 3505 - 3514. PubMed Russo BC, et al. 2019. MBio. 10:3. PubMed Lowery J, et al. 2016. Mol Biol Cell. 27: 608 - 616. PubMed Tiwari A, et al. 2016. Sci Rep. 6:38681. PubMed Heinrich A, et al. 2020. Cell Rep. 31:107513. PubMed Losenkova K, et al. 2020. J Cell Sci. 133:00:00. PubMed Okuyama K, et al. 2018. Int J Oncol. 53:1713. PubMed
RRID: AB_2565208 (BioLegend Cat. No. 919101)
Cell Type: Mesenchymal Stem Cells, Neural Stem Cells
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Intermediate Filaments
Gene ID: 7431
UniProt: View information about Vimentin on UniProt.org
Clone: Poly29191
Regulatory Status: RUO
Other Names: CTRCT30, HEL113, epididymis luminal protein 113
Previously: Covance Catalog# PCK-594P
Isotype: Chicken IgY