Biolegend, 919403, Purified anti-human CD243 (MDR-1) Antibody, 100μg

CD243 (MDR-1) belongs to the ATP binding cassette (ABC) transporter family. With an approximate molecular mass of 170 kD, it consists of two homologous halves. Each half contains two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs). The TMDs span the membrane six times, forming a chamber with a 12 transmembrane α-helix structure. NBDs drive the transport process through ATP coupling and hydrolysis and are located at the cytoplasmic face of the membrane. CD243 transports various molecules across cellular membranes and is involved in multidrug resistance. MDR-1 is expressed on hematopoietic stem cells, T cells, B cells, and NK cells as well as on many multidrug resistant neoplastic cells. CD243 interacts with Caveolin, RING finger protein 1B, AAP1, p53, Orphan nuclear receptor PX, and cytochrome P450.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: SW-1573 cells selected by resistance on doxorubicin.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICC, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Arceci RJ, et al. 1993. Cancer Res. 53:310. Arceci RJ, et al. 1992. Blood 80:1528-36. Schinkel AH, et al. 1993. Int. J. Cancer 55:478. Huet S, et al. 1998. Cytometry 34(6):248-56. Müller MR, et al. 2000. Int. J. Clin. Pharmacol. Ther. 38:180-6. Den Boer ML, et al. 1997. Leukemia 11:1078-85.
Product Citations: Simonneau C, et al. 2021. Fluids Barriers CNS. 18:43. PubMed
RRID: AB_2571901 (BioLegend Cat. No. 919403)
Structure: Member of ABC transporter family with an approximate molecular weight of 170 kD. Consists of two homologous halves, each comprising two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs).
Distribution: Hematopoietic stem cells, T and B cells, natural killer (NK) cells, and many multidrug resistant neoplastic cells.
Function: Transports various molecules across cellular membranes and is involved in multidrug resistance.
Cell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells
Biology Area: Cancer Biomarkers, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: Dulucq S, et al. 2008. Blood 112:2024. Gottesman MM, et al. 2009. Nat. Biotechnol. 27:546. Andreadis C, et al. 2007. Blood 109:3409.
Gene ID: 5243
UniProt: View information about CD243 on UniProt.org
Clone: 4E3.16
Regulatory Status: RUO
Other Names: MDR1, Multidrug resistance protein 1, P-glycoprotein 1, colchicin sensitivity, doxorubicin resistance, ABCB1
Isotype: Mouse IgG2a