Biolegend, 921203, Purified anti-Insulin Degrading Enzyme (IDE) Antibody, 25μg

Insulin degrading enzyme (IDE), also known as insulinase, insulin protease or insulysin, cleaves the peptide hormone insulin with consequences for insulin response and resistance. Since its discovery, IDE has been shown to degrade a variety of bioactive peptides. Recently the finding that IDE clears intracellular and extracellular amyloid products has spurred research into the link between IDE function and human neurodegenerative disease such as Alzheimer’s.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was raised against purified human erythrocyte IDE
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 9B12.225 has been shown to detect a ~118 kD isoform in peripheral tissue and a second isoform at ~54 kD in brain tissue.
Application References(PubMed link indicates BioLegend citation): RG Bennett, et al. 2000. Endocrinology. 141:2508. (WB) Chou YH, et al. 2009. FASEB J. 23:3734. (ICC) PubMed
RRID: AB_2728611 (BioLegend Cat. No. 921203) AB_2728612 (BioLegend Cat. No. 921204)
Structure: IDE is a 1019 amino acid protein with a molecular weight of ~ 118 kD.
Distribution: Tissue distribution: Abundantly expressed in gastrointestinal tract, muscle and nervous system tissues. Cellular distribution: Secreted, mitochondrion, peroxisome, nucleus, cytosol, and plasm membrane.
Function: IDE cleaves the peptide hormone insulin with consequences for insulin response and resistance. IDE is also the enzyme responsible for degradation and clearance of amyloid beta in the brain.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family: Enzymes and Regulators, Proteases
Antigen References: Tang WJ. 2016. Trends Endocrinol Metab. 27(1):24. Jha NK, et al. 2015. J Alzheimers Dis. 48(4):891.
Gene ID: 3416
UniProt: View information about Insulin Degrading Enzyme on UniProt.org
Clone: 9B12.225
Regulatory Status: RUO
Other Names: Abeta-degrading protease, Insulin protease, Insulinase, Abeta-Degrading Protease
Isotype: Mouse IgG1, κ