Biolegend, 927901, Retrieve-All Antigen Unmasking #1: Universal 100X (Previously Covance catalog# SIG-31910), 50ml

50ml
Formulation: Antigen Unmasking Solution.
Storage & Handling: Store at room temperature.
Application: IHC
Recommended Usage: The optimal antigen unmasking condition is a function of temperature (90 to 100°C) and incubation time (5-30 minutes) and should be established by each investigator. The Retreive-All working solution should be prepared by mixing 10 mL of 100x Retrieve-All into 990mL of water. Protocol 1. Deparaffinize sections, rehydrate through graded alcohols, DI water, into PBS for 5 min. 2. Preheat Retrieve-All buffer to 92°C*. 3. Place slides into the preheated Retrieve-All buffer. 4. Incubate for 10 minutes at 92°C. 5. Remove the container with slides and let it cool at room temperature for 5-10 minutes. 6. Rinse in distilled water or PBS. 7. Proceed with usual immunohistochemical staining.
Application Notes: *Concentrated buffers (100x) may show minimal precipitation after long term storage without any effect to the HIER. Retrieve-All is an antigen un-masking solution available in the following three pH formulas for use in heat induced un-masking procedures: Retrieve-All 1 (Universal pH 8) Retrieve-All 2 (Basic pH 10) Retrieve-All 3 (Acidic pH 4.8)
Gene ID: NA
Regulatory Status: RUO
Other Names: Retrieve-All Antigen Unmasking Solution
Previously: Signet Catalog# 1910Covance Catalog# SIG-31910